Lactadherin Competition Assay
FC analysis showing FVIII expression after in vitro lactadherin overnight incubation with U937 cells. Experiments were performed in parallel conditions with/without lactadherin. (A) FVIII surface staining; indicated are means ± SD of n = 4, p = 0.0039 (paired t test). (B) FVIII intracellular staining; indicated are means ± SD of n = 3, in triplicate, p = 0.69. Percentage of IgG background staining (here and in the figures below, if not otherwise specified) is subtracted from the rate of positive cells. (C) Representative dot plots of surface staining after competition assay of lactadherin.