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Double Staining of A2 and C1 Domains of FVIII
GMA® 8012 IgG1 anti A2 domain and GMA® 8011 IgG2a anti C1 domain were used with IgG1 and IgG2a as controls for a comparison of single and double staining on HECV un-transduced cells (A), HECV cells transduced at MOI 30 (B) and 40 (C) with PGK-FVIII-LVV. The average of 2 experiments performed in duplicate is summarized in the graph, with IgG subtracted values (D).
