Supplemental Figure 2.

The expression of hepatic PURB and Adcy6 was regulated by glucagon and fasting. (A) Primary hepatocytes were prepared from C57BL/6 males (8 weeks) and treated with glucagon for different times (0, 1, 2, 4, and 6 h). The expression of PURB and Adcy6 was measured by RT-qPCR (n = 4-5). (B) C57BL/6 males (8 weeks) were fasted for different times (0, 6, 12, and 24 h). The expression of PURB and Adcy6 was measured by RT-qPCR (n = 5). *p < 0.05.