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. 2019 Nov 16;31:85–97. doi: 10.1016/j.molmet.2019.11.008

Figure 8.

Figure 8

Purβ directly binds to the Adcy6 promoter and increases its transcription. (A) Nascent Adcy6 mRNA levels were measured by nuclear run-on RT-qPCR in Purβ-overexpressing hepatocytes (n = 3). (B) ChIP assays showing Purβ occupancy at the promoter of Adcy6 (n = 3). (C) Mouse Adcy6 promoter (−2001 to −1 or −1001 to −1) luciferase reporter plasmids and β-galactosidase reporter plasmids were transiently cotransfected with Purβ expression plasmids into HEK293T cells. Luciferase activity was measured 24 h after transfection and normalized to βGal levels (n = 6). *p < 0.05. **p < 0.01.