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. 2019 Dec 12;10:1617. doi: 10.3389/fpls.2019.01617

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Copyright © 2019 Calabrese, Cusant, Sarazin, Niehl, Erban, Brulé, Recorbet, Wipf, Roux, Kopka, Boller and Courty

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Quantification by qRT-PCR of the transcript abundances of ammonium transporters in R. irregularis. Transcript abundances were measured under low-P and high-P availability. Transcript abundances of the three transporters GintAMT1 (A), GintAMT2 (B), and GintAM3 (C) were measured in the extra-radical mycelium (ERM) and in the intraradical mycelium (IRM) when associated to the host plants poplar and sorghum. Values are means of three biological and three technical replicates. Translational elongation factor was used as a reference transcript. Statistical analysis was performed by analysis of variance for each gene, followed by Tuckey honest significant difference test (Tuckey HSD; p < 0.05). Lower case letters indicate significant difference(Tukey’s t-test; p < 0.05).