Figure 1.

Nuclear and Cytoplasmic miRNAs Microarrays

(A) Subcellular fractionation followed by cytoplasmic marker ACTB mRNA and nuclear marker U6 RNA detection by qRT-PCR; n = 3. ACTB was enriched in cytosol, whereas U6 was highly expressed in the nucleus. (B) Heatmap of miRNA microarray performed in cytoplasmic and nuclear fractions from H9c2 cardiomyocytes. (C) Volcano plot of miRNA profiles in cytoplasmic and nuclear fractions from H9c2 cardiomyocytes. (D) Expression of miR-320, miR-30c-5p, and miR-21-3p in the nucleus relative to cytoplasm by microarray; n = 3, t test was used to compare miRNA levels in the cytosol group and nucleus group. These miRNAs were highly expressed both in the cytosol and nucleus. (E) Representative image of miR-320 in human heart section by in situ hybridization. Scale bar: 20 μm.