Fig. 3.

Expression of pSTAT3 and RUNX1 in Wt and iKspPkd1^del mice after injury and during cyst progression. a Representative immunohistochemistry of Wt and iKspPkd1^del kidneys at 1 week after DCVC (+ injury) or PBS (– injury). Mice without injury showed only sporadic expression of pSTAT3 in the nuclei of tubular epithelial cells (asterisks); after injury, the expression was markedly increased both in Wt mice and in iKspPkd1^del mice. RUNX1 expression in non-injured kidney was present only in some interstitial cells (arrowheads); after injury, RUNX1 was visible in the nuclei of the epithelial cells. b Representative immunohistochemistry of Wt and iKspPkd1^del kidneys at 10 weeks after DCVC (“10weeks”; left and middle panel) showed expression of pSTAT3 and RUNX1 in nuclei in cyst-lining epithelia, in the epithelial cells of surrounding dilated tubules (arrows) and in infiltrating cells (arrowheads) only in cystic tissue. Expression of pSTAT3 and RUNX1 was even more increased at kidney failure (“KF”; right panel) when the kidneys are severely cystic. Scale bars 50 μm