Fig. 6.
Increased binding of STAT3 and RUNX1 to the promoter of target genes in cystic kidneys, shown by ChIP-qPCR. ChIP-qPCR analysis of end-stage renal disease iKspPkd1del kidneys or Wt kidneys at 24 weeks after DCVC. a We confirmed an increased enrichment for STAT3 binding at target genes in iKspPkd1del kidneys compared to Wt kidneys. b RUNX1 enrichment at its targets is not detected in Wt samples (no difference between RUNX1 ChIP and IgG ChIP) but detected in iKspPkd1del samples. Black bars pSTAT3 or RUNX1 antibody, grey bars isotype IgG control (rIgG, rabbit IgG; mIgG, mouse IgG). The Y-axis shows the input-normalised binding-enrichment of the TFs to the indicated genomic region. Data represent the mean of two independent ChIPs ± SD; Two-way ANOVA with Tukey’s multiple comparisons test. *P value < 0.05; **P value < 0.01; ***P value < 0.001