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. 2019 Dec 12;9:1396. doi: 10.3389/fonc.2019.01396

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Copyright © 2019 Yang, Liu, Cheng, Wang, Feng, Yan, Liu and Zhang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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RIZ1 expression was downregulated in cervical cancer tissues compared with the adjacent non-tumor tissues. (A) Representative photomicrographs of RIZ1 immunohistochemical staining. Panel 1, positive RIZ1 expression in cervical squamous cell carcinoma; panel 2, positive RIZ1 expression in cervical cervical adenocarcinoma; panel 3, positive RIZ1 expression in normal cervical tissue; panel 4, negative RIZ1 expression in cervical squamous cell carcinoma; panel 5, negative RIZ1 expression in cervical cervical adenocarcinoma; panel 6, negative RIZ1 expression in normal cervical tissue. Original magnification, ×200. (B) Total proteins from four pairs of cervical cancer tissues (T) and the adjacent non-tumor tissues (N) were extracted for immunoblotting of RIZ1 with β-actin as a loading control. (C) Relative expression RIZ1 to β-actin was quantitated by ImageJ. (D) Cervical cancer tissues (T) and the adjacent non-tumor tissues (N) were extracted for quantitative real-time RT-PCR analysis of RIZ1 mRNA with β-actin as an internal control.