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. 2019 Dec 12;9:1396. doi: 10.3389/fonc.2019.01396

Figure 5.

Figure 5

Overexpression of RIZ1 inhibited the proliferation of cervical cancer cells. (A) HeLa cells and SiHa cells were transfected with plasmid pcDNA3.1-RIZ1-AE or the empty vector pcDNA3.1. 48 h post-transfection, total proteins from each cell lines were extracted for immunoblotting of RIZ1 with β-actin as a loading control. (B) Relative expression RIZ1 to β-actin was quantitated by ImageJ. (C) HeLa cells were transfected with plasmid pcDNA3.1-RIZ1-AE or the empty vector pcDNA3.1. The cell viability on day 1 to day 5 after transfection was assessed by MTT assay. (D) SeHa cells were transfected with plasmid pcDNA3.1-RIZ1-AE or the empty vector pcDNA3.1. The cell viability on day 1 to day 5 after transfection was assessed by MTT assay. Each point indicates the mean of spectrometric absorbance ± SD of three independent experiments. *P < 0.05, **P < 0.005.