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. 2019 Dec 12;9:1396. doi: 10.3389/fonc.2019.01396

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Copyright © 2019 Yang, Liu, Cheng, Wang, Feng, Yan, Liu and Zhang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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RIZ1 overexpression induced cell cycle arrest at the G2-M phase and apoptosis of cervical cancer cells. HeLa cells and SiHa cells were transfected with plasmid pcDNA3.1-RIZ1-AE or the empty vector pcDNA3.1 for 48 h. (A) Cell cycle distribution was assessed with PI staining followed by flow cytometry. (B) Percentage of cells at different phases, (C) Cell apoptosis was assessed with Annvin V staining followed by flow cytometry, (D) Percentage of Annexin V positive cells. The data are the mean ± SD of one representative experiment. Similar results were obtained in three independent experiments.