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. 2019 Nov 22;31:109–123. doi: 10.1016/j.molmet.2019.11.013

Figure 6.

Figure 6

Expression of Bdnf-e1 in BAT-connected neurons in LH. (A–D) Immunostaining images showing the co-expression of Bdnf-e1–driven eGFP (green) and PRV-614-mRFP (red) in LH (C-D) but not in PVH (A-B). PRV-614-mRFP was injected into the left BAT of Bdnf-e1−/− mice at 3 months of age. These mice were processed for immunostaining 6 days after injection. Coronal sections of frozen brain were used for double-staining of eGFP and mRFP. (B) and (D) are the enlarged confocal immunostaining images (scale bar, 50 μm) in the white rectangular areas of (A) and (C), respectively. (E) Percentage of Bdnf-e1–eGFP neurons positive for PRV infection (left), and percentage of PRV-positive neurons stained for Bdnf-e1–driven eGFP (right) in the LH. Note that ∼90% of thermogenesis-related neurons express Bdnf-e1 transcript.