Figure 7.

NMJ Morphology and CMAPs Are Impaired in MCT4^−/− Mice

(A) Representative images of whole-mount immunohistochemistry of EDL muscles labeling motor axons (SMI32, green), pre-synaptic nerve terminals (SV2, green) and post-synaptic endplate (rhodamine-conjugated α-bungarotoxin, red). I., intact; P.D., partially denervated; F.D., fully denervated; M.I., multiple innervated.

(B) Quantification of intact and disrupted NMJs in EDLs of 12-month-old MCT4^−/− and MCT4^+/+ (n = 3 each group, Chi-square, p < 0.0001).

(C) Representative CMAP trace showing the electromyographic signal recorded from gastrocnemius muscle (black trace) upon stimulation of the sciatic nerve (green trace). Amplitude and RMS are indicated.

(D) CMAPs RMS (Root-Mean-Square) recorded at 20 Hz in 6-month-old MCT4^−/− and MCT4^+/+ mice (n = 4 each group, Student t test, *p < 0.05). All the data are rapresented as mean and SEM.

See also Figure S3