Figure 5.

Overexpression of IFI44 Could Mimic the Effect of si-LINC01116 on the Sensitivity of PC9/R Cells to Gefitinib

(A) Quantitative real-time PCR detection of IFI44 mRNA in stably transfected empty vector or pcDNA-IFI44 PC9/R cells. (B) Western blotting detection of IFI44 protein expression in stably transfected empty vector or pcDNA-IFI44 PC9/R cells. (C) MTT analysis of the IC₅₀ values of gefitinib in PC9/R/NC or PC9/R/IFI44 cells. (D) Colony-formation assays of proliferation in PC9/R/NC or PC9/R/IFI44 cells combined with gefitinib (5 μmol/L). (E) Flow cytometric analysis of apoptosis in PC9/R/NC or PC9/R/IFI44 cells combined with gefitinib (5 μmol/L). (F) Flow cytometric analysis of cell cycle in PC9/R/NC or PC9/R/IFI44 cells combined with gefitinib (5 μmol/L). Data are expressed as the mean ± SD of three individual experiments. *p < 0.05; **p < 0.01.