Scgn is expressed in primary and immortalized murine and human L-cells. (A) RNA-seq analysis of Scgn in murine Gcg-Venus L-cells and Venus-negative cells from duodenal, ileal, and colonic sections (n = 2–3 for each cell population). (B) RT-qPCR for Scgn in murine colonic Gcg-Venus L-cells, Venus-negative cells, and mGLUTag L-cells (n = 3 for each cell population). (C) RNA-seq analysis for SCGN in human jejunal L-cells, enteroendocrine cells, and non-endocrine intestinal epithelial cells (n = 11 for each cell population). (D-G) Immunostaining of murine (D) and human (E) ileal sections for SCGN and GLP-1 (representative images of SCGN+:GLP-1+ (top) and SCGN+:GLP-1- (bottom) cells are shown). Cell count histogram for murine (F) and human (G) ileal SCGN and/or GLP-1 stained cells (n = 5 sections for each species; a total of ∼100 cells were counted per section). (H-I) Microarray analysis of mGLUTag cells (n = 6) (H) and RNA-seq analysis of hNCI-H716 cells (n = 2) (I) for proglucagon, clock, and SNARE protein transcripts. (J-K) Immunostaining of mGLUTag (J) and hNCI-H716 (K) L-cells for SCGN; DAPI shows the nuclear stain (representative images of n = 4 are shown). *p < 0.05, **p < 0.01, ***p < 0.001.