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Time-dependent binding of BMAL1 to the Scgn promoter in mGLUTag L-cells. (A) Three noncanonical BMAL1 E-boxes were identified at 1252, 1176, and 672 base pairs upstream of the transcription start site. (B) ChIP analysis for the BMAL1 binding sites in the Scgn promoter at 4, 8, 16, and 20 h in synchronized mGLUTag L-cells (n = 6, conducted as 3 replicates from each of 2 independent splits). *p < 0.05; ###p < 0.001 vs negative control (-IgG).
