Figure 6.

Time-dependent recruitment of SCGN to the cell membrane in mGLUTag L-cells. (A-B) 8 h and (C-D) 20 h after synchronization, mGLUTag L-cells were treated with 10⁻⁷ M GIP or vehicle for 0, 10, and 60 min, followed by staining for SCGN and a membrane marker (with wheat germ agglutinin). Co-localization of SCGN with the cell membrane was determined by Pearson's correlation coefficient. (n = 4, conducted as 2 replicates from each of 2 independent splits; representative images are shown). *p < 0.05.