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Time-dependent interactions of SCGN with β-actin but not SNAP25 in mGLUTag L-cells. mGLUTag L-cells were synchronized and then 8 or 20 h later treated with 10−7 M GIP or vehicle for 2 h. (A) Immunoprecipitation of SNAP25 and immunoblotting for SCGN. (B) Immunoprecipitation of SCGN and immunoblotting for β-actin. (n = 4, conducted as 2 replicates from each of 2 independent splits; representative blots are shown). *p < 0.05.
