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. 2019 Dec 18;9:19377. doi: 10.1038/s41598-019-55848-7

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Impact of Na₂S₄ on death of mouse MIN6 cells. (a,b) MIN6 cells were exposed to Na₂S₄ at doses from 0 µM to 100 µM and to lidocaine as positive control at 10 mM and cultured for periods ranging from 0 h to 4 h prior to cell viability evaluation. Cell death was also assayed by flow cytometry (a) and trypan blue exclusion method (b). Differences between treatments were evaluated by one-way ANOVA followed by Dunnett’s test for multiple comparisons; ^#P < 0.05, as compared to the control cell population (Na₂S_{4 ;}0 µM, lidocaine; 0 mM treatment).