Figure 6.

Effects of polysulfide salts on insulin secretion induced by glibenclamide. (a,b) Mouse MIN6 cells were exposed to Na₂S₄ (a) or Na₂S₃ (b) (0, 6.2, 12.5, 25, or 50 µM) for 4 h without or with glibenclamide (10 µM) under 2 mM or 20 mM glucose conditions (n = 3). (c) MIN6 cells were exposed to 60 mM K⁺ with Na₂S₄ (n = 3). Insulin secretion was determined as described in Materials and Methods. Data are presented as the mean ± SD (n = 4). Differences between treatments were evaluated by one-way ANOVA followed by Dunnett’s test for multiple comparisons; ^#P < 0.05, as compared with the control (glucose = 2 mM, without glibenclamide or without diazoxide treatment or without high K⁺ treatment).