Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Dec 18;10:5774. doi: 10.1038/s41467-019-13810-1

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 7 — a Schematics of zero-mode waveguide (ZMW)-based single-molecule FRET assay to monitor translation. Elongation factors including fluorescently-labeled tRNA (Phe-(Cy5)-tRNA^Phe) and quencher-labeled large ribosomal subunit (BHQ-50S) are delivered to pre-initiation complex (PIC) with labeled small ribosomal subunit (Cy3B-30S) and mRNA tethered to the ZMWs. b Expected fluorescence signal observed from a translating complex utilizing FRET between Cy3B and BHQ-2 on ribosomal subunits, as well as direct excitation of Cy5 on Phe-(Cy5)-tRNA^Phe. c Measured percentage of complete translation events for different codon 3–5 mRNA constructs (n = 179 molecules for all; error bars represent s.e. based on the binomial distribution). d Representative traces for “processive” translation of K₃I₄H₅ (Left) and “abortive” translation of T₃V₄G₅ (Right).