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. 2019 Dec 18;10:5779. doi: 10.1038/s41467-019-13593-5

Fig. 5. EC-HA+ mice as an in vivo model for CTL-mediated endotheliopathy.

Fig. 5

a Investigation of T cell CNS infiltration in the brain, retina, and inner ear of EC-HA− or EC-HA+ recipient mice on day 7 after adoptive transfer. Left: Representative histological sections documenting T cell infiltration (CD3+, brown). Right: Quantification of tissue-infiltrating T cells in EC-HA− (closed blue circles) or EC-HA+ (closed cayenne diamonds) recipient mice (n = 4–8 per group); data for retina and inner ear originate from 2 independent experiments, involving transfer of cytotoxic CD8+ T cells alone or with control IgG. b Quantification of the cytolytic activity of CNS-infiltrating T cells analyzed as CD107a expression on day 7 after adoptive transfer in CD45.2+ EC-HA− (closed blue circles) or EC-HA+ recipient mice (closed cayenne diamonds) (n = 7–8 per group, 2 independent experiments). c Weight loss (left; 11–12 mice per group) and rotarod motor performance (right; 7–8 mice per group) following adoptive transfer of HA-specific CTLs in EC-HA− (closed blue circles) or EC-HA+ (closed cayenne diamonds) mice. Data are from three (body weight) or two (motor performance) independent experiments. Statistical analysis was using the unpaired Student’s t test (a, b) or two-way ANOVA with Bonferroni post-test (c), respectively. Error bars indicate the mean ± s.d.; p values: *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001. Source data are provided as a Source Data file.