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. 2019 Dec 18;9:19321. doi: 10.1038/s41598-019-55836-x

Figure 1.

Figure 1

Experimental protocol for OVA-induced asthma model mice and intratracheal administration of Schizophyllum commune. Mice were divided into four groups: OVA-sensitized/challenged and intratracheally administered S. commune (OVA/Sc), OVA-sensitized/challenged and intratracheally administered PBS (OVA/PBS), non-sensitized and intratracheally administered S. commune (PBS/Sc), and non-sensitized and intratracheally administered PBS (PBS/PBS). Mice were intraperitoneally sensitized with OVA on day −15 and day −10, then challenged via exposure to aerosolized OVA on days −3 and −2. Mice in the OVA/Sc and PBS/Sc groups were intratracheally administered a S. commune mycelial suspension on day 0. All animals were euthanized 24 h after the intratracheal administration of S. commune.