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. 2019 Dec 18;9:19379. doi: 10.1038/s41598-019-54259-y

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Viability of HCAECs after repeated treatments with live-P. gingivalis (A) and P. gingivalis-LPS (B). The HCAECs were stimulated to repeated live-P. gingivalis (MOI 1:100 - 1:0,1) and P. gingivalis-LPS (1.0, 3.5 and 7.0 µg/mL) exposures, during 24 h. Cell viability was determined according to the fluorometric detection after reduction of resazurin in the resorufin product using AlamarBlue. 1% was considered our positive control of cell death. Percentage of cell viability with respect to the control. *Represents the statistical difference with respect to the control or without stimulus. (p < 0.05). Three independent experiments were performed; the results are presented as the means ± SEM (n = 3).