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. 2019 Dec 18;10(12):954. doi: 10.1038/s41419-019-2187-8

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — Kaplan–Meier plotter online tools were used to identify that the patients suffered from breast cancer with low TLR5 expression had a significantly worse survival rate (a). CCK-8 assay showed that TLR5⁻ 4T1 cells have higher proliferation ability than TLR5⁺ 4T1 cells (b).No difference in apoptosis change in TLR5 downregulated 4T1 cells was detected compared with negative control group (c). The colony formation ability of TLR5⁻ 4T1 cells was significantly increased than TLR5⁺ 4T1 cells (d).The migration area of TLR5⁻ 4T1 group was larger than TLR5⁺ 4T1 group (e). The number of migrated cells was remarkably reduced in TLR5⁺ 4T1 group than TLR5⁻ 4T1 group (f). The data were presented as the means ± SD (n = 3) from three independent experiments, analyzed by Student’s t test. *P < 0.05, **P < 0.01.