Fig. 3. TLR5 downregulation promoted lung metastasis of 4T1 in vivo.

Fluorescence images of stripped lungs of mice models: naive 4T1 (no Lentivirus transfection), TLR5⁺ 4T1 (with negative Lentivirus transfection) and TLR5⁻ 4T1 (with Lentivirus-shRNA TLR5 knockdown transfection) (a). Lungs from untreated mice, TLR5⁺ 4T1 and TLR5⁻ 4T1 model mice were weighed (b) and H&E staining was performed (c) (n = 3, **P < 0.01). Representative results of three independent experiments were reported. The data were presented as the means ± SD. The data were analyzed by Student’s t test. *P < 0.05, **P < 0.01.