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. 2019 Dec 18;9:19388. doi: 10.1038/s41598-019-56045-2

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Experimental set-up. (A) Full thickness surgical resection specimen of Crohn’s disease patients with a stricturing disease phenotype were obtained from the stenotic segment and the adjacent, non-stenotic segment of the intestinal wall. (B) Experimental setup for off-axis digital holographic microscopy (DHM) and bright field imaging; (C) Bright field image of representative stenotic tissue; (D) corresponding digital off-axis hologram; (E) quantitative phase image reconstructed from the digital hologram in D; (F) enlarged part of the digital hologram that illustrates the off-axis carrier fringes; (G) false color coded pseudo 3D representation of the quantitative phase image in E.