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. 2019 Dec 18;9:19388. doi: 10.1038/s41598-019-56045-2

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Significant correlation between the refractive index (RI) determined by digital holography microscopy (DHM) and the immunofluroescence-intensity of fibrosis markers. (A–C) The RI of the intestinal tissue (mucosa, submucosa and muscularis propria) determined by DHM in 30 surgical resection specimens significantly correlated with the light intensity of fibrosis markers determined by immunofluorescence studies (all p < 0.001). (D) The strongest correlation was found between the RI and the average immunofluorescence-intensity of all three fibrosis markers (p < 0.001; R² = 0.477). Statistical analysis was performed using Pearson-correlation. Two-sided p values < 0.05 were considered statistically significant.