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. Author manuscript; available in PMC: 2020 Feb 13.
Published in final edited form as: Lab Invest. 2019 Aug 13;100(1):38–51. doi: 10.1038/s41374-019-0297-7

Figure 1. Growth of CR-NB cells is associated with cytoskeleton remodeling.

Figure 1

A. Primary NB cells isolated from a tumor arising in TH-MYCN mice, cultured without ROCK inhibitor, stained with Alexa-Fluor 488 phalloidin. B. Phalloidin-stained CR-NB cell line developed from TH-MYCN tumor in the presence of ROCK inhibitor, Y-27632. Fluorescence microscopy, scale bar represents 100 μm. C. Growth curve of primary CR-NB cells in optimized cell culture conditions: CR F medium + 10 μM Y-27632 compound, non-coated vessels, 2% oxygen.