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. Author manuscript; available in PMC: 2020 Feb 13.
Published in final edited form as: Lab Invest. 2019 Aug 13;100(1):38–51. doi: 10.1038/s41374-019-0297-7

Figure 7. Malignant features of CR-NB cell lines.

Figure 7

A-B. Subsequent stages of the anchorage-independent growth of CR-NB cells on poly-HEMA low attachment plates. C-D. Cells grown in suspension on poly-HEMA plates for 7 days were plated into standard cell culture plates under established optimal culture conditions (C) and upon reaching confluency visualized by methylene blue staining (D). E-F. Cells cultured for 7 days under anchorage-independent conditions in methylcellulose were plated into the standard culture plates and their growth assessed as above. Scale bars represent 400 μm. G. CR-NB cells invade the extracellular matrix 4 days after embedding. Scale bar represents 200 μm H. CR-NB cell lines showed a significant motility in a transwell migration assay. Mean fluorescence intensity ± standard error is shown. *** represent p<0.001 as compared to control (no cells).