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. Author manuscript; available in PMC: 2021 Jan 1.
Published in final edited form as: J Immunol. 2019 Dec 4;204(1):137–146. doi: 10.4049/jimmunol.1900721

Figure 1. Dgkζ deficiency ameliorates SCW-induced arthritis.

Figure 1.

C57Bl/6 WT and Dgkζ-/- mice were injected with 25 μg of SCW fragments in the right ankle while the left ankle injected with PBS served as control, and the animals were euthanized 3 days later. (A) Representative images of ankles from WT and Dgkζ-/- mice injected with PBS or SCW fragments. The joint swelling in WT and Dgkζ-/- mice was determined by caliper measurement of the injected ankles. (B) Representative histological sections of ankle joints stained with H&E showing presence of immune infiltrates. (C) Representative images of ankle joints stained with safranin O depicting cartilage destruction. (D-E) RT-PCR analysis for the immune markers CD11b, F4/80 and CD3 (D) and inflammatory cytokines IL-6, TNFα and IL-1β (E) in the ankle joints of WT and Dgkζ deficient mice. Bars in Figure A and D-E (n= 3 mice/group) represent mean and SD. Statistical significance was determined by two-tailed Student’s t test or two-way ANOVA for multiple comparison. * p<0.05, **p<0.01, ***p<0.001.