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. Author manuscript; available in PMC: 2021 Jan 1.
Published in final edited form as: J Immunol. 2019 Dec 4;204(1):137–146. doi: 10.4049/jimmunol.1900721

Figure 5. Loss of Dgkζ limits M1 macrophage polarization.

Figure 5.

(A) The transcript levels of iNOS, CCL5 and IRF5 determined by RT-qPCR analysis in BMMs stimulated with 100 ng/ml of LPS for 4 hr. Data are expressed as fold change in gene expression in comparison to unstimulated WT BMMs. (B) Nitrite concentration in the supernatant of BMMs stimulated with LPS (100 ng/ml) and IFNʏ (100 ng/ml) determined by Griess assay. (C) BMMs stimulated with IL-4 for indicated time and subjected to western blot analysis for phospho-STAT6 and STAT6. β-Actin was used as a loading control. (D) RT-qPCR analysis of Arginase 1 and PPARʏ in BMMs derived either from WT or Dgkζ-/- mice and stimulated with 50 ng/ml of IL-4 for 12 hr. Data are expressed as fold change in gene expression in comparison to unstimulated WT BMMs. Statistical significance was determined by two-way ANOVA. * p<0.05, **p<0.01, ***p<0.001.