Figure 6. Inflammatory conditions induce Dgkζ expression.
(A) Expression of Dgkζ was determined by RT-qPCR in BMMs left either unstimulated (Ctrl) or stimulated with LPS (100 ng/ml), CpG (1 μM) and IL-4 (50 ng/ml) for 12 hr. (B) mRNA expression of Dgkζ determined in the liver of mice that were given repeated injections of PBS or CpG to induce CSS (n=5/ group). (C) mRNA expression of Dgkζ measured in BMMs treated with either healthy control or sJIA plasma for 12 hr. Bars in Figure A and B represents mean and SD while each symbol in Figure C represent 1 individual with horizontal lines representing the mean values. Statistical significance was determined using unpaired t-test or one-way ANOVA. * p<0.05, **p<0.01, ***p<0.001.