Figure 5.

Stability study of HBCL1 (just the linker, not the full conjugate) as a function of time. The ¹H and ¹³C NMR (400 MHz, DMSO_d6) spectra of HBCL1 are shown at (a) day 0 and (b) day 90. The cross-linker was stored in DI water during the 90-day period. He and Cg peaks are denoted by an asterisk. Figure S5 provides complete labeling of the spectra. (c) The ¹H and ¹³C NMR (400 MHz, DMSO_d6) spectra of SH-PEG₇-COOH.