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. Author manuscript; available in PMC: 2021 Apr 1.
Published in final edited form as: Mol Imaging Biol. 2020 Apr;22(2):367–376. doi: 10.1007/s11307-019-01386-7

Figure 2.

Figure 2.

Site-specific Zr-89 radiolabeling of A2 cys-diabody. a The A2cDb is reduced under mild conditions and conjugated site-specifically with maleimide-DFO followed by chelation of Zr-89. b SDS-PAGE analysis shows unconjugated A2cDb (left lane) migrating at ~50 kDa. The A2cDb-malDFO migrates as monomer confirming site-specific conjugation. c Size exclusion chromatography shows similar elution profiles for A2cDb and A2cDb-malDFO, confirming that malDFO conjugation does not impair the non-covalent dimeric conformation of the diabody.