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. Author manuscript; available in PMC: 2021 Apr 1.
Published in final edited form as: Shock. 2020 Apr;53(4):476–484. doi: 10.1097/SHK.0000000000001394

Figure 3: Norepinephrine evokes specific branches of the ER stress pathway.

Figure 3:

A) Representative immunostaining of cleaved ATF6 in HepG2 cells treated with 100 μM norepinephrine and the alpha-1, alpha-2, and beta-blocker (100 μM) (prazosin, yohimbine and propranolol, respectively) for 24 hours. B) Quantification of cleaved-ATF6 positive (+) cells obtained from the immunostaining performed in (A). C) qPCR expression profile of XBP-1s in HepG2s exposed to norepinephrine (100 μM) alone or in presence of the alpha- and beta-blockers (100 μM) for 24 hours. NE, Norepinephrine. Arrows indicate positive cells. Experiments were repeated 3 separate times with a total sample size (n=8–9) per group included in the analysis. *p<0.05 vs NE or CTL respectively.