Figure 4: Norepinephrine does not induce CHOP and apoptosis.
A) Immunostaining of CHOP (Green) in HepG2 cells treated for 24 hours with norepinephrine (NE, 100 μM) or Thapsigargin (1.5 μM). Arrows indicate CHOP-positive cells. B) TUNEL assay was performed on HepG2 cells treated in the same conditions described in A). Arrows indicate TUNEL-positive cells. C) Averaged relative levels of cGMP in HepG2 treated with norepinephrine (100 μM) alone or in presence of the alpha- and beta-blockers (100 μM) for 24 hours. Experiments were repeated 3 separate times with a total sample size (n=9) per group included in the analysis.** p<0.01, *** p<0.001 vs CTL. ND, Not detected.
