Figure 4. CggR intracellular levels and expression profile with different promoters, strains, and conditions.

1. CggR intracellular abundance in the wild‐type (WT) strain on glucose strongly varies with the promoter used. The CggR intracellular levels were quantified by proteomics in steady‐state cultures grown in minimal media with glucose as carbon source at a final concentration of 10 g/l. Error bars represent the standard deviation of at least three replicate experiments.
2. The relative abundance of CggR (normalized to the abundance measured on glucose and the same promoter) is almost constant with P_TEFmut7 across multiple growth rates in WT and TM6 cells, but not with P_TEFmut2 and P_CMV. The CggR intracellular levels were quantified by proteomics in steady‐state cultures grown in minimal media with glucose, galactose, maltose, or pyruvate as carbon sources at a final concentration of 10 g/l. WT data include all three promoters, whereas TM6 only includes the P_TEFmut2 and P_TEFmut7 data. Error bars represent the standard deviation of at least three replicate experiments.