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. 2017 Oct 11;24(1):1549–1564. doi: 10.1080/10717544.2017.1386729

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© 2017 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — Intracellular localization of GE11-Se NPs. (A) Intracellular trafficking of coumarin-6-loaded GE11-Se NPs. KYSE-150 cells treated with coumarin-6-loaded GE11-Se-NPs (80 μM) were stained with lysotracker (lysosome) or different periods of time and visualized under a confocal microscopy, scale bar: 20 μm. The changes in integrity of the lysosomal membrane (AO staining) in KYSE-150 cells after 24 h GE11-Se-NPs (80 μM) by (B) confocal microscopy and (C) flow cytometry, scale bar; 30 μm. (D) Proposed endocytosis pathway of GE11-Se-NPs in KYSE-150 cells.