Table 2.
The primer sequences used for quantitative real time PCR.
| Gene Name | Primer Sequence (5‘–3′) | GenBank |
|---|---|---|
| claudin-1 | F: CATACTCCTGGGTCTGGTTGGT | AY750897.1 |
| R:GACAGCCATCCGCATCTTCT | ||
| occludin | F:ACGGCAGCACCTACCTCAA | D21837.1 |
| R:GGGCGAAGAAGCAGATGAG | ||
| mucin-2 | F:TTCATGATGCCTGCTCTTGTG | XM_421035 |
| R:CCTGAGCCTTGGTACATTCTTGT | ||
| IFN-γ | F:AGCTGACGGTGGACCTATTATT | Y07922 |
| R:GGCTTTGCGCTGGATTC | ||
| TLR2 | F:CTGGGAAGTGGATTGTGGA | AB050005.2 |
| R:AAGGCGAAAGTGCGAGAAA | ||
| TLR4 | F:AGTCTGAAATTGCTGAGCTCAAAT | NM-001030693 |
| R:GCGACGTTAAGCCATGGAAG | ||
| NF-κB | F:GTGTGAAGAAACGGGAACTG | NM-205129 |
| R:GGCACGGTTGTCATAGATGG | ||
| MyD88 | F:CTGGCATCTTCTGAGTAGT | NM-001030962 |
| R:TTCCTTATAGTTCTGGCTTCT | ||
| GAPDH | F:CCTAGGATACACAGAGGACCAGGTT | NM-204305 |
| R:GGTGGAGGAATGGCTGTCA |