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. 2019 Nov 6;9(11):712. doi: 10.3390/biom9110712

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Effect of melatonin on SNAT enzyme activity. (A) A schematic of the enzymic reaction. (B) Melatonin-mediated inhibition of NAS synthesis by recombinant AtSNAT1. (C) Melatonin-mediated inhibition of NAS synthesis by recombinant AtSNAT2. AtSNAT1 was prepared as described previously [28]. The SNAT enzyme assay was performed in the presence of 0.5 μg AtSNAT2, 0.5 mM serotonin, and various levels of melatonin. The data are the means of three replicates. Asterisks (*) denote significant differences compared to the Col-0 controls (Student’s t-test; p < 0.05).