Figure 1.

Schematic diagram of the experimental design. Vascular endothelial cells (EC) (EA.hy926) were stably transfected by a eukaryotic expression vector containing human secretory leukocyte protease inhibitor (SLPI) mRNA (hspli) (A). The stable cells overexpressing recombinant human SLPI were used for further experiments. Determination of the paracrine effect of endothelial-derived SLPI on cardiomyocyte (CM) (H9c2) subjected to hypoxia/reoxygenation (H/R) injury was performed via techniques following two different principles: co-culture between SLPI-overexpressing EA.hy926 cells and H9c2 cells by the Transwell culture system (B), and conditioned medium from SLPI-overexpressing EA.hy926 cells (C).