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. 2019 Nov 12;11(11):600. doi: 10.3390/pharmaceutics11110600

Figure 1.

Figure 1

(A) Reaction scheme of tyrosine-coupling onto branched polyethylenimines (PEIs). Abbreviations: TFA, Trifluoroacetic acid; NHS, N-Hydroxysuccinimid; EDC, 1-Ethyl-3-(3-dimethylaminopropyl) carbodiimid (B) Knockdown efficacies of various tyrosine-modified PEI/siRNA complexes in PC3 cells targeting the oncogenes polo-like kinase 1 (PLK1) (left) or survivin (right), quantitated by RT-qPCR. (C) Determination of anchorage-dependent proliferation of PC3 cells upon transfection with the tyrosine-modified PEI/siRNA complexes using different anti-proliferative siRNAs at the amounts indicated in the figures. SiRNA-mediated cell inhibition is compared to negative-control transfected or untreated cells. (D) Knockdown upon transfecting Saos-2 cells with siRNAs against PLK1 or survivin, as determined by RT-qPCR. Increasing mass ratios from 2.5 to 3.75 does not further improve the knockdown efficacy. (E) Determination of anchorage-dependent proliferation of Saos-2 cells upon transfection with P5Y/siRNA complexes targeting PLK1 or survivin. The statistical significance indicates the difference to negative-control transfected cells. Left: quantitation based on WST-8 measurements; right: original pictures.