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(A) Determination of the hydrodynamic diameters and zeta potentials of P5Y/siRNA complexes at different mass ratios as indicated in the figure. (B) Size measurement of P5Y/siRNA complexes by nanoparticle tracking (NTA) at the optimal mass ratio of 2.5. (C) Luciferase knockdown efficacies in H441-Luc cells for P5Y/siRNA complexes pre-incubated with increasing amounts of FCS (v/v) prior to transfection for 1 h (left) or upon storage for 4 weeks at 4 °C.
