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. 2019 Oct 29;11(11):561. doi: 10.3390/pharmaceutics11110561

Figure 1.

Figure 1

High-Performance Liquid Chromatography (HPLC) setup with a fluorescence detector (excitation: 345 nm, emission: 470 nm) for the analysis of bile acids. The Schematic diagram of bile acids analysis system shows the Waters® 2695 Alliance HPLC system equipped with analysis components (1 through 11). The flow direction through column sets (7. Separation column, 10. Enzyme reaction column) is indicated by arrows. The flow from the component 7 (separation column) is merged with flow from the component 9 (reagent pump), and then the mixtures of mobile solvents with the separated eluents and reagent solution comes through the reaction coil into the Enzymepakcolumn (component 10). Finally, the flow from the Enzyme column goes into the fluorescence detector (component 11).