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. 2019 Nov 1;11(11):572. doi: 10.3390/pharmaceutics11110572

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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NPs delivering an equivalent of 100 μM KLVFF peptides are non-toxic for neurons. Toxicity profile of ethanol (positive control), CNT-NPs, 100 μM free KLVFF peptide, and NPs delivering 100 μM KLVFF peptide. Neuronal cells were treated for 24 h and compared to untreated control. Apoptotic cells were identified using Annexin V labeled with FITC (Fluorescein isothiocyanate), necrotic cells by ethidium homodimer III, and the total number of cells was assessed using Hoechst 33342, labeling all nuclei. A total of three optic fields of view per condition was analyzed. No significant effect of any treatment except ethanol was found for neuronal cell health (one-way ANOVA followed by Tukey test). Exemplary images for Control and positive Control (EtOH) is shown in the right panel.