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. 2019 Nov 11;9(11):724. doi: 10.3390/biom9110724

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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(a) Graphical representation of IMP-1 (PDB Code 1DD6 [8]) with Zn(II) shown as gray spheres and Zn(II) ligands in loop 7 as thin sticks. The two residues mutated in this study, S115 and S119, are shown as thick sticks labeled in red. Note that S119 is shown in two alternative conformations as per the crystal structure. The figure was generated with VMD [14] Version 1.9.3. The backbone is shown as a cyan cartoon. Residues are colored by atom: C, gray; N, blue; O, red. (b) Phylogenetic tree of the currently known IMP-type variants. Variants containing the S115T and S119G mutations studied here are highlighted in yellow, the IMP-1 reference enzyme in blue, and variant IMP-46 harboring S115T in combination with S119D in green. The tree was generated with MEGA [15] Version 7.