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(a) Representative Western blot of the four different IMP variants expressed in E. coli DH10B cells. DnaK was used as a constitutively expressed marker. The different IMP variants were labeled with a previously reported anti-IMP-1 antibody [22,23]. Exposure times were optimized to obtain good signals without saturation. (b) Quantification of three different Western blot experiments. Only the expression level of IMP-1-S119G was significantly lower than that of IMP-1 (p < 0.01).
