Figure 1.

Localization of the cadavers of blackbirds (Turdus merula) and song thrushes (T. philomelos) collected in 2017–2019 in the three geographical locations in the Czech Republic (indicated in black in the overview map of Europe) and used for detection of Usutu virus (USUV). (a) Localization of the three main sampling sites (cities of Prague, Brno and Ceske Budejovice) and localization of the three additional USUV positive samples collected outside the area of the three cities are depicted in the map of the Czech Republic. The region of South Moravia is labeled by a blue background and the region of South Bohemia by green. Bird pictogram indicates an USUV positive blackbird, collected in the town of Breclav-South Moravia); mosquito pictograms indicate USUV positive mosquito pools (pool of Cx. modestus collected near Hlohovec-South Moravia in yellow, Culex pipiens collected near Lomnice nad Luznici-South Bohemia in red). Position of cadavers of blackbirds and song thrushes collected in the three cities are presented in the remaining maps: Prague (b), Ceske Budejovice (c), Brno (d). Blackbirds negative for USUV genomic RNA are indicated in black, USUV positive blackbirds in red, and song thrushes (all USUV negative) in violet.