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. 2019 Nov 8;11(11):587. doi: 10.3390/pharmaceutics11110587

Figure 1.

Figure 1

mRNA expression fold-change between BMEC and bEnd.3 cells, the latter used as the calibrator. Both cell monolayers were either grown in simple DMEM medium (a) or in specialized BMEC medium (b). Relative gene expression was calculated using the 2−ΔΔCt method, with HPRT as the housekeeping gene and the bEnd.3 cell line as the calibrator. Bars indicate the average mRNA fold change ± SD (n = 3). Data were analyzed using an unpaired t test with Welch’s correction (non-statistically significant (ns): p ≥ 0.0332, * p < 0.0332, ** p < 0.0021, *** p < 0.0002).